A: No it does not matter. The object of RotStop is to protect (what we term) the leak pathways. Leaks allow unwanted water to enter behind the cladding and into the timber framing. When a leak happens, water can get behind the cladding and be transported via gravity and absorbency pathways to much larger areas. This also occurs with RotStop during injection.
What is important is that if a leak occurs in the future, water will pass the RotStop injection points and dissolve some of the boron thus tainting the water as it spreads, killing all rot fungi in the process. We target and deposit concentrated RotStop in the leak pathways where it will be useful as the dwelling ages.